Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells

被引：13
|
作者：
Laparra, Jose Moises [1 ]
Tako, Elad [1 ]
Glahn, Raymond P. [2 ]
Miller, Dennis D. [1 ]
机构：
[1] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA
[2] USDA, ARS, Plant Soil & Nutr Lab, Ithaca, NY 14853 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 08期
关键词：
prebiotic; inulin; iron bioavailability; iron uptake; ferritin; Caco-2; cells;
D O I：
10.1021/jf073470m
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The in vitro effects of inulin on the fluxes of Fe (F-Fe) and uptake by Caco-2 cells from FeSO4 and FeEDTA were evaluated. Cell ferritin formation was used as a measure of Fe uptake. Mitochondrial (MTT test) and lysosomal activities were monitored as biomarkers of the changes of cellular metabolism. Changes in mRNA expression of Fe transporters, DMT1 and Dcytb, were evaluated. Inulin decreased dialyzability and F-Fe from FeSO4 solution, suggesting a mineral binding effect, but increased those from FeEDTA. Cultures exposed to FeEDTA solutions exhibited higher ferritin values and MTT conversion percentages. Regardless of Fe source, cell Fe uptake and mRNA expression of Fe transporters were similar with or without inulin, suggesting that inulin did not impair Fe uptake. These observations might indicate a faster cellular Fe internalization from FeEDTA solutions. From a physiological perspective, the decreased F-Fe from FeSO4 might be reflected in a decreased Fe uptake.
引用
收藏
页码：2846 / 2851
页数：6
相关论文
共 40 条
  • [1] Iron uptake by Caco-2 cells from NaFeEDTA and FeSO4:: Effects of ascorbic acid, pH, and a Fe(II) chelating agent
    Zhu, Le
    Glahn, Raymond P.
    Yeung, Chi Kong
    Miller, Dennis D.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (20) : 7924 - 7928
  • [2] Comparison of iron uptake from reduced iron powder and FeSO4 using the Caco-2 cell model:: Effects of ascorbic acid, phytic acid, and pH
    He, Wan-Ling
    Feng, Ying
    Li, Xiao-Li
    Yang, Xiao-E
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (08) : 2637 - 2642
  • [3] IRON STATUS AFFECTS ALUMINUM UPTAKE AND TRANSPORT BY CACO-2 CELLS
    ALVAREZHERNANDEZ, X
    MADIGOSKY, SR
    STEWART, B
    GLASS, J
    JOURNAL OF NUTRITION, 1994, 124 (09): : 1574 - 1580
  • [4] Uptake of iron from ferritin by Caco-2 cells
    Lonnerdal, B
    Kelleher, SL
    Theil, EC
    FASEB JOURNAL, 2004, 18 (05): : A767 - A767
  • [5] Effect of milk peptides that enhance iron uptake by Caco-2 cells on the expression of DMT-1 and on iron dialyzability from meals
    Kapsokefalou, Maria
    Argyri, Konstantina
    Tako, Elad
    Glahn, Raymond P.
    Miller, Dennis D.
    FASEB JOURNAL, 2008, 22
  • [6] Iron uptake from iron chelates and iron salts by Caco-2 cells
    Yeung, CK
    Glahn, RP
    Miller, DD
    FASEB JOURNAL, 2004, 18 (04): : A155 - A155
  • [7] REDUCTION OF FE(III) IS REQUIRED FOR UPTAKE OF NONHEME IRON BY CACO-2 CELLS
    HAN, O
    FAILLA, ML
    HILL, AD
    MORRIS, ER
    SMITH, JC
    JOURNAL OF NUTRITION, 1995, 125 (05): : 1291 - 1299
  • [8] Bioavailability of zinc from infant foods by in vitro methods (solubility, dialyzability and uptake and transport by Caco-2 cells)
    Perales, S
    Barberá, R
    Lagarda, MJ
    Farré, R
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2006, 86 (06) : 971 - 978
  • [9] Mossbauer, FT-IR and FE SEM investigation of iron oxides precipitated from FeSO4 solutions
    Gotic, Marijan
    Music, Svetozar
    JOURNAL OF MOLECULAR STRUCTURE, 2007, 834 : 445 - 453
  • [10] Varying media zinc, iron, and calcium affects uptake and transport of cadmium in Caco-2 cells
    Reeves, PG
    FASEB JOURNAL, 2004, 18 (04): : A528 - A528
1234