This paper analyzes the building process of the main greenhouse gas (GHG) emissions (CO2, CH4 and N2O) inventory from the energy sector in Palestine. The paper includes determination tools, i.e., emission factors, to estimate the amounts of national GHG emissions from sub-sectors of energy including energy industries, manufacturing industries and construction, transport and other sectors (households, agriculture and commerce and public services). The results show that the total amount of national GHG emissions from the energy sector in 2016 was 4131 thousand metric tons of CO2-equivalent (TtCO(2e)), which represented 0.011% of the total global GHG emissions. The average value of GHG emissions per capita from the energy sector was 0.86 tCO(2e) in Palestine, and its gross domestic product was estimated at 3212 $/ton of CO2e. The estimated amounts of CO2, CH4 and N2O emission from the energy sector were 4022, 49 and 60 TtCO(2e), respectively. The transport and household sub-sectors dominated the national GHG emissions from the entire energy sector by 58 and 32%, respectively. In general, fuels including diesel, gasoline, wood and charcoal and liquefied petroleum gas made most of the total amount of the national GHG emissions from the energy sector at 50, 18, 18 and 12%, respectively. Finally, the mitigation actions included in the first nationally determined contribution of Palestine and recommendations to help lower the national GHG emissions from the Palestinian energy sector are provided.