Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

被引：9

作者：

Danh, Kenneth ^{[1
]}

Karp, Donna Grace ^{[1
]}

Singhal, Malvika ^{[1
]}

Tankasala, Akshaya ^{[1
]}

Gebhart, David ^{[1
]}

Cortez, Felipe de Jesus ^{[1
]}

Tandel, Devangkumar ^{[1
]}

Robinson, Peter, V ^{[1
]}

Seftel, David ^{[1
]}

Stone, Mars ^{[2
]}

Simmons, Graham ^{[2
]}

Bagri, Anil ^{[3
]}

Schreiber, Martin A. ^{[4
]}

Buser, Andreas ^{[5
]}

Holbro, Andreas ^{[5
]}

Battegay, Manuel ^{[6
,7
]}

Morris, Mary Kate ^{[8
]}

Hanson, Carl ^{[8
]}

Mills, John R. ^{[9
]}

Granger, Dane ^{[9
]}

Theel, Elitza S. ^{[9
]}

Stubbs, James R. ^{[9
]}

Corash, Laurence M. ^{[3
]}

Tsai, Cheng-ting ^{[1
]}

机构：

[1] Enable Biosci Inc, San Francisco, CA 94080 USA

[2] Vitalant Res Inst, San Francisco, CA USA

[3] Cerus Corp, Concord, CA USA

[4] Oregon Hlth & Sci Univ, Dept Surg, Portland, OR 97201 USA

[5] Univ Basel, Univ Hosp Basel, Reg Blood Transfus Serv, Swiss Red Cross, Basel, Switzerland

[6] Univ Basel, Univ Hosp Basel, Div Infect Dis, Basel, Switzerland

[7] Univ Basel, Univ Hosp Basel, Hosp Epidemiol, Basel, Switzerland

[8] Calif Dept Publ Hlth, Richmond, CA USA

[9] Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USA

来源：

NATURE COMMUNICATIONS | 2022年 / 13卷 / 01期

关键词：

VALIDATION; PLASMA;

D O I：

10.1038/s41467-022-31796-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91-97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern. Neutralizing antibodies are critical for conferring immunity against SARS-CoV-2. Here, Dahn et al. report a PCR assay termed SONIA (Split-Oligonucleotide Neighboring Inhibition Assay) for measuring neutralizing antibodies against multiple SARS-CoV-2 strains in fingerprick dried blood spot samples.

引用

页数：8

共 50 条

[1] Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR
Kenneth Danh
Donna Grace Karp
Malvika Singhal
Akshaya Tankasala
David Gebhart
Felipe de Jesus Cortez
Devangkumar Tandel
Peter V. Robinson
David Seftel
Mars Stone
Graham Simmons
Anil Bagri
Martin A. Schreiber
Andreas Buser
Andreas Holbro
Manuel Battegay
Mary Kate Morris
Carl Hanson
John R. Mills
Dane Granger
Elitza S. Theel
James R. Stubbs
Laurence M. Corash
Cheng-ting Tsai
[J]. Nature Communications, 13
[2] Detection of SARS-CoV-2 IgG antibodies in dried blood spots
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