Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

被引：4

作者：

Paolo, Dario ^{[1
]}

Locatelli, Franca ^{[1
]}

Cominelli, Eleonora ^{[1
]}

Pirona, Raul ^{[1
]}

Pozzo, Sara ^{[1
]}

Graziani, Giulia ^{[2
]}

Ritieni, Alberto ^{[2
]}

De Palma, Monica ^{[3
]}

Docimo, Teresa ^{[3
]}

Tucci, Marina ^{[3
]}

Sparvoli, Francesca ^{[1
]}

机构：

[1] CNR, Inst Agr Biol & Biotechnol CNR IBBA, Via Edoardo Bassini 15, I-20133 Milan, Italy

[2] Univ Naples Federico II UNINA, Dept Pharm, Via Domenico Montesano 49, I-80131 Naples, Italy

[3] CNR, Inst Biosci & Bioresources CNR IBBR, Via Univ 133, I-80055 Portici, Italy

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 21期

关键词：

plant cell cultures; lignin; cellulose accessibility; nutraceuticals; MYB4; CYNARA-CARDUNCULUS L; POSSIBLE ALTERNATIVE UTILIZATION; BIOMASS PRODUCTION; LIGNIN BIOSYNTHESIS; CHLOROGENIC ACID; OIL PRODUCTION; ARTICHOKE; GRAIN; ENERGY; GROWTH;

D O I：

10.3390/ijms222111978

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefaciens-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the Arabidopsis thaliana MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of AtMYB4 overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

引用

页数：24