GC-MS screening of phenylketonuria in newborns

被引：0

作者：

Deng, CH ^{[1
]}

Hu, YM

Hu, KJ

Zhang, YF

Gu, XF

机构：

[1] Fudan Univ, Ctr Anal & Measurement, Shanghai 200433, Peoples R China

[2] Xinghua Hosp, Shanghai 200082, Peoples R China

来源：

ACTA CHIMICA SINICA | 2001年 / 59卷 / 01期

关键词：

phenylketonuria; amino acid; screening; GC; -; MS; derivation;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Phenylalanine(Phe) and Tyrosine(Tyr) were extracted from dry blood spots with 0.1% mol/L HCl - methanol. The proteins in the extraction were removed by centifuging. Phe and Tyr were treated with 3.0 mol/L HCl - butanol. Phe and Tyr butyl esters were treated with trifluoro acetic anhydride. The derivatives from Phe and Tyr were analyzed by GC - MS. The contents of the two amino acids in blood samples were determined by calculating the areas of their derivatives with reference to an exterior standard. Finally the diagnosis of PKU was made on the basis of the molar content ratios of Phe to Tyr.

引用

页码：133 / 136

页数：4