Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid

被引：116
|
作者：
Kugimiya, A [1 ]
Takeuchi, T [1 ]
机构：
[1] Hiroshima City Univ, Fac Informat Sci, Lab Synthet Biochem, Asaminami Ku, Hiroshima 7313194, Japan
来源：
BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期
关键词：
surface plasmon resonance; molecular imprinting; sialic acid; ganglioside; biomimetic sensor;
D O I：
10.1016/S0956-5663(01)00227-5
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：1059 / 1062
页数：4
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