Culture media on in vitro development of castor bean (Ricinus communis L.) stem tips

The research of the culture medium composition suitable for plant species and the type of explant to be used is the most significant factor in plant tissue culture. The culture of stem tips with plant donor recovery is a technique of great impact for the in vitro plant production, regeneration of virus-free plants, germoplasm conservation and genetic modification. The objective of this study was to evaluate culture media composition in vitro on direct organogenesis of the FCA-UNESP-PB population of castor bean (Ricinus communis L.), from stem tips for clonal propagation of elite genotypes. Four formulations were tested: basic MS (T1), modified MS 1 (T2), modified MS 2 (T3) and WPM (T4) in a completely randomized experimental design with 20 replicates pertreatment with 1stem tip/flask. The treatment T3was superior, and differed significantly from the other treatments, with 35% of stem tips developed, followed by 10% in T2. T1 and T4 showed no plant tissue differentiation. The results allowed to conclude that the balance of mineral salts and culture media evaluated especially the rate NO3 / NH4 and the absence of FeSO4 center dot 7H(2)O showed nigh influence on the development of stem tips of castor bean.