Intramolecular cross-linking of proteins with azobenzene-based cross-linkers

被引：4
|
作者：
Yasuike, Nobuo [1 ,2 ]
Lu, Huixin [1 ]
Xia, Peng [1 ]
Woolley, G. Andrew [1 ]
机构：
[1] Univ Toronto, Dept Chem, Toronto, ON, Canada
[2] JSR Corp, Tokyo, Japan
来源：
OPTOCHEMICAL BIOLOGY | 2019年 / 624卷
关键词：
PHOTO-CONTROL; PHOTOSWITCHES; RED; TRIS(2-CARBOXYETHYL)PHOSPHINE; DESIGN; LIGHT;
D O I：
10.1016/bs.mie.2019.04.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The photo-control of protein activity can often be achieved via the photo-control of protein structure. Intramolecular cross-linkers that change length upon photoisomerization provide a means to photo-control protein structure by linking to pairs of Cys residues in a protein sequence. In this protocol, we describe general methods for introducing intramolecular cross-linkers, both UV light switchable and red-light switchable, under either denaturing or native conditions.
引用
收藏
页码：129 / 149
页数：21
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