A simple method for isothermal titration calorimetric data analysis

被引:0
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作者
Saboury, AA [1 ]
机构
[1] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran
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中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A simple method was introduced for isothermal titration calorimetric data analysis. This method was applied to the binding of monosaccharide methyl alpha -D-mannopyranoside to concavalin A at pH 6.9 and temperature of 25 degreesC. The dissociation binding constant (K) and the molar enthalpy of binding (DeltaH) were 0.135 mM end -28.47 kJ/mol, respectively. These results were markedly consistent with the results obtained from previous method.
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页码:204 / 208
页数:5
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