Corynebacterium guangdongense sp nov., isolated from a contaminated plate

A novel Gram-reaction-positive, non-motile and facultatively anaerobic bacterium, designated strain S01(T), was isolated from a nutrient agar plate kept on a laboratory clean bench at Guangdong Institute of Microbiology, PR China, which was contaminated from an unknown source. Strain S01(T) was found to be catalase-positive and oxidase-negative. Similarity searches revealed that the strain shared the highest 16S rRNA gene similarity with Corynebacterium humireducens MFC-5(T) (95.9 %). However, phylogenetic analysis based on the 16S rRNA gene sequences showed that strain S01(T) was closely related to Corynebacterium doosanense JCM 17317(T) (94.8 %) and Corynebacterium maris JCM 17018(T) (94.8 %). The major fatty acids were C-18: 1 omega 9c, C-16: 0, 10-methyl C-18: 0 and C-18: 0. The respiratory quinones predominantly consisted of MK-8(H-2), with small amounts of MK-8 and MK-9(H-2). Polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unidentified aminolipid, two unidentified glycolipids and two unidentified lipids. Mycolic acids were present. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major cell-wall sugars were galactose, arabinose and glucose. The genomic DNA G+C content of strain S01(T) was 70.7 +/- 0.1 mol%. The results of phenotypic, phylogenetic and chemotaxonomic analyses indicated that strain S01(T) represents a novel species of the genus Corynebacterium, for which the name Corynebacterium guangdongense sp. nov. is proposed. The type strain is S01(T) (=GDMCC 1.1022(T) =CCTCC AB 2015423(T) =KCTC 39608(T)).