Development of nodes of Ranvier in feline nerves: An ultrastructural presentation

The ultrastructure of developing nodes of Ranvier and adjacent paranodes of future large myelinated fibers in feline lumbar spinal roots is described. The development starts before birth concurrent with myelination and is finished at the end of the first postnatal month when the nodal regions of future large fibers, now 4-5 mu m of diameter, for the first time appear like miniatures of those of their 4 times thicker and fully mature counterparts. At this stage the fibers also begin to show mature functional properties. The latent maturation process is denoted ''nodalization'' and includes two major events: (1) the formation of a narrow node gap bordered by compact myelin segments and filled with Schwann cell microvilli that interconnect an undercoated nodal axolemma with rapidly increasing accumulations of mitochondria lodging in the longitudinal cords of Schwann cell cytoplasm that is distributed outside a more and more crenated paranodal myelin sheath; (2) the setting of a fixed number of nodes along the axons; an event that includes segmental axonal and myelin sheath degeneration and is concluded by the elimination of supernumerary Schwann cells. (C) 1996 Wiley-Liss, Inc.