Protective effects of scoparone against lipopolysaccharide-induced acute lung injury

The purpose of this study was to investigate the protective effects and molecular mechanisms of scoparone on lipopolysaccharide (LPS)-induced acute lung injury in mice. Mice model of acute lung injury (ALI), induced by intranasal instillation of LPS, was used to investigate the protective effects of scoparone in vivo. The alveolar macrophages were used to investigate the molecular mechanisms of scoparone in vitro. The results showed that scoparone treatment remarkably attenuated LPS-induced pulmonary edema, histological severities, myeloperoxidase activity, and TNF-alpha, IL-6 and IL-1 beta production in vivo. We also found that scoparone inhibited LPS-induced TLR4 expression, NF-kappa B activation, TNF-alpha, IL-6 and IL-1 beta production in alveolar macrophages in vitro. In conclusion, our results suggest that scoparone has a protective effect on LPS-induced ALI via suppression of TLR4-mediated NF-kappa B signaling pathways. (C) 2014 Elsevier B.V. All rights reserved.