Structure and gene cluster of the O-antigen of Escherichia coli O19ab

被引:4
|
作者
Perepelov, Andrei V. [1 ]
Zhu, Hongfei [2 ]
Senchenkova, Sofya N. [1 ]
Wang, Quan [2 ]
Shashkov, Alexander S. [1 ]
Wang, Lei [2 ]
Knirel, Yuriy A. [1 ]
机构
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA, TEDA Sch Biol Sci & Biotechnol, Tianjin 300071, Peoples R China
基金
美国国家科学基金会; 俄罗斯基础研究基金会;
关键词
Escherichia coli; Bacterial polysaccharide structure; O-antigen; Lipopolysaccharide; O-antigen gene cluster; L-RHAMNOSE; OVEREXPRESSION; IDENTIFICATION; ORGANIZATION; PURIFICATION;
D O I
10.1016/j.carres.2011.09.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The O-polysaccharide (O-antigen) of Escherichia coli O19ab was studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established: -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-D-Glcp-(1 -> 3)-alpha-D-GlcpNAc6Ac-(1 -> where the degree of O-acetylation of GlcNAc is similar to 33%. The O-antigen gene cluster of E. coli O19ab was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O19ab-antigen structure. (C) 2011 Published by Elsevier Ltd.
引用
收藏
页码:2812 / 2815
页数:4
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