Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes

被引:6
|
作者
Park, Jae Kyun [1 ,2 ]
Lee, Ju Hee [1 ]
Park, Eun A. [3 ]
Lim, Hyunjung J. [4 ]
Lyu, Sang Woo [2 ]
Lee, Woo Sik [2 ]
Kim, Jayeon [3 ]
Song, Haengseok [1 ]
机构
[1] CHA Univ, Dept Biomed Sci, Seongnam 13488, South Korea
[2] CHA Univ, CHA Fertil Ctr Gangnam, Seoul 06125, South Korea
[3] CHA Univ, CHA Fertil Ctr Seoul Stn, Seoul 04637, South Korea
[4] Konkuk Univ, Sch Vet Med, Dept Vet Med, Seoul 05029, South Korea
基金
新加坡国家研究基金会;
关键词
oocyte vitrification; closed carrier systems; cross-contamination; cooling rate; warming rate; exposure time to cryoprotectants; viability; developmental competence; SLUSH NITROGEN VITRIFICATION; FERTILITY PRESERVATION; CRYOPRESERVATION; EMBRYOS; EXPERIENCE; EFFICIENCY; EXPOSURE; WOMEN;
D O I
10.3390/cells10071670
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and developmental competence of vitrified/warmed (VW) oocytes using the CC were significantly lower than with OC. We aimed to improve the efficiency of the CC. Metaphase II oocytes were collected from mice after superovulation and subjected to in vitro fertilization after vitrification/warming. Increasing the cooling/warming rate and exposure time to cryoprotectants as key parameters for the CC effectively improved the survival rate and developmental competence of VW oocytes. When all the conditions that improved the outcomes were applied to the conventional CC, hereafter named the modified vitrification/warming procedure using CC (mVW-CC), the viability and developmental competence of VW oocytes were significantly improved as compared to those of VW oocytes in the CC. Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future.
引用
收藏
页数:14
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