Label-Free Indirect Immunoassay Using an Avidin-Induced Surface-Enhanced Raman Scattering Substrate

被引：33
|
作者：
Han, Xiao Xia [1 ,2 ]
Chen, Lei [3 ]
Ji, Wei [3 ]
Xie, Yunfei [3 ]
Zhao, Bing [3 ]
Ozaki, Yukihiro [1 ,2 ]
机构：
[1] Kwansei Gakuin Univ, Sch Sci & Technol, Dept Chem, Sanda, Hyogo 6691337, Japan
[2] Kwansei Gakuin Univ, Sch Sci & Technol, Res Ctr Single Mol Vibrat Spect, Sanda, Hyogo 6691337, Japan
[3] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
来源：
SMALL | 2011年 / 7卷 / 03期
关键词：
PREPARED SILVER SURFACE; SPECTROSCOPY; ANTIGEN; ADSORPTION; SERS; RECOGNITION; MECHANISMS; PEPTIDES; PROTEINS; BINDING;
D O I：
10.1002/smll.201001936
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A label-free immunoassay based on a simple, surface-enhanced Raman scattering (SERS) approach is reported. Biotin-conjugated antibodies are attached to avidin-aggregated Ag nanoparticles via biotin-avidin biorecognition. More highly sensitive protein detection and more biocompatible protein adsorption are achieved compared to previous relevant methods. Importantly, a SERS intensity ratio of two bands is found to be sensitive to the concentration of corresponding antigens. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：316 / 320
页数：5
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