GYRA AND GYRB MUTATIONS IN OFLOXACIN-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES IN THAILAND

被引：0
|
作者：
Pitaksajjakul, Pannamthip [1 ,2 ]
Worakhunpiset, Suwalee [1 ]
Chaiprasert, Angkana [3 ]
Boonyasopun, Jirakarn [4 ]
Ramasoota, Pongrama [1 ,2 ]
机构：
[1] Mahidol Univ, CEAR, Fac Trop Med, Bangkok 10400, Thailand
[2] Mahidol Univ, Dept Social & Environm Med, Fac Trop Med, Bangkok 10400, Thailand
[3] Mahidol Univ, Siriraj Hosp, Fac Med, Bangkok 10700, Thailand
[4] Minist Publ Hlth, Chest Dis Inst, Nonthaburi, Thailand
来源：
SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH | 2011年 / 42卷 / 05期
关键词：
Mycobacterium tuberculosis; ofloxacin-resistant; mutation; gyrA; gyrB; MULTIDRUG EFFLUX PUMP; FLUOROQUINOLONE RESISTANCE; QUINOLONE RESISTANCE; SELECTION; MUTANTS;
D O I：
暂无
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
In order to identify mutations in gyrA and gyrB genes in 92 ofloxacin-resistant Mycobacterium tuberculosis (OFXr-MTB) clinical isolates collected from Siriraj Hospital, Mahidol University and Chest Disease Institute, Thailand. The quinolone resistance-determining regions (QRDR) of gyrA and gyrB in all 92 OFXr-MTB isolates were amplified using polymerase chain reaction and sequenced. There were 70 isolates with point mutations associated with ofloxacin resistance. In gyrA QRDR, 69 isolates had mutations in gyrA Gly88 (Ala/(75), Ala90 (Val), Ser91 (Pro) and Asp94 (Gly/Ala/His/Asn), the latter being the most common (42%). Only one isolate was found with mutation at position Asp495 (Asn). The other 22 isolates had no mutations in both gyrA and gyrB QRDR. Thus, point mutations in gyrA and gyrB QRDR were responsible for OFXr-MTB clinical isolates in Thailand.
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页码：1163 / 1167
页数：5
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