UV-Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory

被引：24
|
作者：
Felton, Daniel E. [1 ]
Ederer, Martina [2 ]
Steffens, Timothy [2 ]
Hartzell, Patricia L. [2 ]
Waynant, Kristopher V. [1 ]
机构：
[1] Univ Idaho, Dept Chem, 875 Perimeter Dr, Moscow, ID 83844 USA
[2] Univ Idaho, Dept Biol Sci, 875 Perimeter Dr, Moscow, ID 83844 USA
来源：
JOURNAL OF CHEMICAL EDUCATION | 2018年 / 95卷 / 01期
关键词：
UV-Vis Spectroscopy; First-Year Undergraduate/General; Interdisciplinary/Multidisciplinary; Inquiry-Based/Discovery Learning; Laboratory Instruction; HERBICIDE GLYPHOSATE; RESISTANT CROPS; SOIL; CHEMISTRY; DEGRADATION; BACTERIA; ADSORPTION; ROUNDUP; IMPACTS; BIOLOGY;
D O I：
10.1021/acs.jchemed.7b00440
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth: A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV-vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.
引用
收藏
页码：136 / 140
页数：5
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