Reproducibility of retention time and peak area in comprehensive two-dimensional liquid chromatography

被引：8
|
作者：
Elsner, Victoria [1 ]
Wulf, Volker [2 ]
Wirtz, Michaela [2 ]
Schmitz, Oliver J. [1 ,3 ]
机构：
[1] Berg Univ Wuppertal, Inst Pure & Appl Mass Spectrometry, D-42119 Wuppertal, Germany
[2] BASF Personal Care & Nutr GmbH, D-40589 Dusseldorf, Germany
[3] Univ Duisburg Essen, D-45141 Essen, Germany
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2015年 / 407卷 / 01期
关键词：
Comprehensive two-dimensional liquid chromatography; LCxLC-MS; Surfactants; HILIC; ALCOHOL ETHOXYLATES; MASS-SPECTROMETRY; SEPARATION; POLYMERS; QUANTIFICATION;
D O I：
10.1007/s00216-014-8090-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Comprehensive two-dimensional liquid chromatography is used to separate anionic, nonionic, and amphoteric surfactants by substance class, alkyl chain distribution, and degree of ethoxylation. A nearly orthogonal system with a hydrophilic interaction chromatography (HILIC) phase in the first and a reversed-phase material in the second dimension is applied to generate a separation with maximum peak capacity. The potential of the developed method is demonstrated by the reproducibility of retention time and peak area, which shows standard deviations less than 5 % and the analysis of real samples. An external calibration and the standard addition method were applied to determine unknown concentrations for the alkyl chain homologues of a betaine and for one ethoxylate (EO) homologue of a fatty alcohol ethoxylate in a sample mixture.
引用
收藏
页码：279 / 284
页数：6
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