A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

被引：15
|
作者：
Goh, Yun Shan [1 ]
Ng, Lisa F. P. [1 ,2 ,3 ,4 ]
Renia, Laurent [1 ,5 ]
机构：
[1] ASTAR, Infect Dis Labs, ID Labs, Immunos,Biopolis, Singapore 138648, Singapore
[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore 117596, Singapore
[3] Univ Liverpool, Natl Inst Hlth Res, Hlth Protect Res Unit Emerging & Zoonot Infect, Liverpool, England
[4] Univ Liverpool, Inst Infect Vet & Ecol Sci, Liverpool, England
[5] Nanyang Technol Univ, Lee Kong Chian Sch Med, 11 Mandalay 20 Rd, Singapore 308232, Singapore
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 03期
基金：
英国医学研究理事会;
关键词：
Antibody; Cell Biology; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Molecular Biology;
D O I：
10.1016/j.xpro.2021.100671
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmis-sion. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein.For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).
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收藏
页数：16
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