KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells

AIM: To investigate the function of the KISS-1 gene in gastric carcinoma cells and to explore its potential mechanism. METHODS: A KISS-1 eukaryotic expression vector was constructed and transfected into BGC-823 cells. Resistant clones were obtained through G418 selection. reverse transcription-polymerase chain reaction and western blotting were used to detect KISS-1 and matrix metalloproteinase-9 (MMP-9) expression in transfected cells. The growth of transfected cells was investigated by 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MIT) proliferation assays, and the cells' invasive potential was analyzed by basement membrane (Matrigel) invasion assays. The anti-tumor effects of KISS-1 were tested in vivo using allografts in nude mice. RESULTS: The expression level of KISS-1 mRNA and protein in BGC-823/KISS-1 transfected cells were significantly higher than in BGC-823/pcDNA3.1 transfected cells (P < 0.05) or the parental BGC-823 cell line (P < 0.05). The expression level of MMP-9 mRNA and protein in BGC-823/KISS-1 were significantly less than in BGC-823/pcDNA3.1 (P < 0.05) or BGC-823 cells (P < 0.05). MU growth assays show the proliferation of BGC-823/KISS-1 cells at 48 h (0.642 +/- 0.130) and 72 h (0.530 +/- 0.164) were significantly reduced compared to BGC-823/pcDNA3.1 (0.750 +/- 0.163, 0.645 +/- 0.140) (P < 0.05) and BGC-823 cells (0.782 +/- 0.137, 0.685 +/- 0.111) (P < 0.05). Invasion assays indicate the invasive potential of BGC-823/KISS-1 cells (16.50 +/- 14.88) is significantly reduced compared to BGC-823/pcDNA3.1 (20.22 +/- 14.87) (P < 0.05) and BGC-823 cells after 24 h (22.12 +/- 16.12) (P < 0.05). In vivo studies demonstrate the rate of pcDNA3.1-KISS-1 tumor growth is significantly slower than pcDNA3.1 and control cell tumor growth in nude mice. Furthermore, tumor volume of pcDNA3.1-KISS-1 tumors (939.38 +/- 82.08 mm(3)) was significantly less than pcDNA3.1 (1250.46 +/- 44.36 mm(3)) and control tumors (1284.36 +/- 55.26 mm(3)) (P < 0.05). Moreover, the tumor mass of pcDNA3.1-KISS-1 tumors (0.494 +/- 0.84 g) was significantly less than pcDNA3.1 (0.668 +/- 0.55 g) and control tumors (0.682 +/- 0.38 g) (P <0.05). CONCLUSION: KISS-1 may inhibit the proliferation and invasion of gastric carcinoma cells in vitro and in vivo through the downregulation of MMP-9. (C) 2012 Baishideng. All rights reserved.