Saliniradius amylolyticus gen. nov., sp. nov., isolated from solar saltern sediment

A novel non-pigmented, Gram-stain-negative, motile by means of a polar flagellum, aerobic and rod-shaped bacterium, designated HMF8227(T), was isolated from solar saltern sediment sampled at Shinan, Republic of Korea. The isolate was able to grow at 15-42 degrees C (optimum, 37 degrees C), at pH 6-8 (pH 7) and with 0.5-12% NaCl (2-5%). Strain HMF8227(T) was positive for hydrolysis of starch and dextrin. 16S rRNA gene sequence analysis revealed that strain HMF8227(T) was affiliated with the family Alteromonadaceae, sharing the highest sequence similarities to the genera Salinimonas (93.0-94.4%), Aestuariibacter (92.0-94.2%), Alteromonas (92.0-93.6%) and Lacimicrobium (93.6%). In the phylogenetic trees, strain HMF8227(T) formed an independent clade with Lacimicrobium alkaliphilum X13M-12(T). The major fatty acids were C-16:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified glycolipids. The DNA G+C content of the genomic DNA was 52.1 mol%. On the basis of the polyphasic characterizations, strain HMF8227(T) represents a novel species and genus within the family Alteromonadaceae, for which the name Saliniradius amylolyticus gen. nov., sp. nov. is proposed, with the type strain being HMF8227(T) (=KCTC 62462(T)=NBRC 113230(T)).