Ferrovibrio terrae sp. nov., isolated from soil

Designated strain K5(T) was isolated from soil on Jeju Island. The bacterium was aerobic, Gram-stain-negative, oxidase-positive, catalase-low activity, motile, short-rod shaped, opaque and formed white colonies that were circular, raised and had entire margins. Strain K5(T) was able to grow at 15-40 degrees C, pH 4-9 and at 0-2% (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain K5(T) is closely related to Ferrovibrio soli A15(T) (98.9% sequence similarity), Ferrovibrio denitrificans Sp-1(T) (98.7%) and Ferrovibrio xuzhouensis LM-6T (97.4%). The sole respiratory quinone was determined to be ubiquinone-10. The dominant fatty acids of strain K5(T) were summed feature 8 (C-18:1 omega 7c / C-18:1 omega 6c. 29.8%), C-19:0 cyclo omega 8c (20.2%) and C-16(:0) (24.4%). DNA G+C content was 63.6% and DNA-DNA relatedness between strain K5(T) and other three members of the genus Ferrovibrio ranged from 24 to 28%. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine and aminolipids. Moreover, polyphasic characterization revealed that strain K5(T) represents a novel species in the genus Ferrovibrio, for which the name Ferrovibrio terrae sp. nov. is proposed. The type strain is K5(T) (=KCCM 43295(T) =LMG 30611(T)).