Scc2 regulates gene expression by recruiting cohesin to the chromosome as a transcriptional activator during yeast meiosis

被引:21
|
作者
Lin, Weiqiang [1 ]
Jin, Hui [1 ]
Liu, Xiuwen [2 ]
Hampton, Kristin [1 ]
Yu, Hong-Guo [1 ]
机构
[1] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA
[2] Florida State Univ, Dept Comp Sci, Tallahassee, FL 32306 USA
基金
美国国家科学基金会;
关键词
SISTER-CHROMATID COHESION; DE-LANGE-SYNDROME; SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; NIPPED-B; FISSION YEAST; S-PHASE; ORGANIZATION; DROSOPHILA; PROTEINS;
D O I
10.1091/mbc.E10-06-0545
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To tether sister chromatids, a protein-loading complex, including Scc2, recruits cohesin to the chromosome at discrete loci. Cohesin facilitates the formation of a higher-order chromosome structure that could also influence gene expression. How cohesin directly regulates transcription remains to be further elucidated. We report that in budding yeast Scc2 is required for sister-chromatid cohesion during meiosis for two reasons. First, Scc2 is required for activating the expression of REC8, which encodes a meiosis-specific cohesin subunit; second, Scc2 is necessary for recruiting meiotic cohesin to the chromosome to generate sister-chromatid cohesion. Using a heterologous reporter assay, we have found that Scc2 increases the activity of its target promoters by recruiting cohesin to establish an upstream cohesin-associated region in a position-dependent manner. Rec8-associated meiotic cohesin is required for the full activation of the REC8 promoter, revealing that cohesin has a positive feedback on transcriptional regulation. Finally, we provide evidence that chromosomal binding of cohesin is sufficient for target-gene activation during meiosis. Our data support a noncanonical role for cohesin as a transcriptional activator during cell differentiation.
引用
收藏
页码:1985 / 1996
页数:12
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