Mouse palmitoyl protein thioesterase: Gene structure and expression of cDNA

被引：11

作者：

Salonen, T

Hellsten, E

Horelli-Kuitunen, N

Peltonen, L

Jalanko, A ^{[1
]}

机构：

[1] Univ Helsinki, Natl Publ Hlth Inst, FIN-00300 Helsinki, Finland

[2] Univ Helsinki, Inst Biomed, Dept Human Mol Genet, FIN-00300 Helsinki, Finland

[3] Univ Helsinki, Cent Hosp, Meilahti Hosp, Mol Genet Lab, FIN-00290 Helsinki, Finland

来源：

GENOME RESEARCH | 1998年 / 8卷 / 07期

关键词：

D O I：

10.1101/gr.8.7.724

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Palmitoyl protein thioesterase (PPT) is the defective enzyme in infantile neuronal ceroid lipofuscinosis (INCL), which is a recessively inherited, progressive neurodegenerative disorder. We present here the cloning, chromosomal mapping, genomic structure, and the expression of the cDNA of mouse PPT. The mouse PPT gene spans >21 kb of genomic DNA and contains nine exons with a coding sequence of 918 bp. Fluorescence in situ hybridization to metaphase chromosomes localized the mouse PPT gene to the chromosome 4 conserved syntenic region with human chromosome 1p32 where the human PPT is located. PPT is expressed widely in a variety of mouse tissues. The mouse PPT cDNA is conserved highly with the human and rat PPT both at the nucleotide and amino acid sequence level. Transient expression of mouse PP7 in COS-1 cells yielded a 38/36-kD differentially glycosylated polypeptide that was also secreted into culture media. Immunofluorescence analysis of transiently transfected HeLa cells indicated lysosomal localization of mouse PPT. Based on the high conservation of the gene and polypeptide structure as well as similar processing and intracellular localization, the function of PPT in mouse and human are likely to be very similar.

引用

页码：724 / 730

页数：7

共 50 条

[1] Expression of palmitoyl protein thioesterase in neurons
Heinonen, O
Kyttälä, A
Lehmus, E
Paunio, T
Peltonen, L
Jalanko, A
MOLECULAR GENETICS AND METABOLISM, 2000, 69 (02) : 123 - 129
[2] Developmental expression of palmitoyl protein thioesterase in normal mice
Isosomppi, J
Heinonen, O
Hiltunen, JO
Greene, NDE
Vesa, J
Uusitalo, A
Mitchison, HM
Saarma, M
Jalanko, A
Peltonen, L
DEVELOPMENTAL BRAIN RESEARCH, 1999, 118 (1-2): : 1 - 11
[3] MOLECULAR-CLONING AND EXPRESSION OF PALMITOYL-PROTEIN THIOESTERASE
CAMP, LA
VERKRUYSE, LA
AFENDIS, SJ
SLAUGHTER, CA
HOFMANN, SL
JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (37) : 23212 - 23219
[4] Expression of a Gossypium hirsutum cDNA encoding a FatB palmitoyl-acyl carrier protein thioesterase in Escherichia coli
Huynh, TT
Pirtle, RM
Chapman, KD
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2002, 40 (01) : 1 - 9
[5] Palmitoyl-protein thioesterase (PPT) expression in developing rat brain
Suopanki, J
Tyynelä, J
Baumann, M
Haltia, M
JOURNAL OF NEUROCHEMISTRY, 1999, 73 : S173 - S173
[6] Palmitoyl-protein thioesterase gene expression in the developing mouse brain and retina: Implications for early loss of vision in infantile neuronal ceroid lipofuscinosis
Zhang, ZJ
Mandal, AK
Wang, N
Keck, CL
Zimonjic, DB
Popescu, NC
Mukherjee, AB
GENE, 1999, 231 (1-2) : 203 - 211
[7] Molecular cloning and expression of palmitoyl-protein thioesterase 2 (PPT2), a homolog of lysosomal palmitoyl-protein thioesterase with a distinct substrate specificity
Soyombo, AA
Hofmann, SL
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) : 27456 - 27463
[8] Primary structure of mouse and rat nephrin cDNA and structure and expression of the mouse gene
Putaala, H
Sainio, K
Sariola, H
Tryggvason, K
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2000, 11 (06): : 991 - 1001
[9] Lysosomal targeting of palmitoyl-protein thioesterase
Verkruyse, LA
Hofmann, SL
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (26) : 15831 - 15836
[10] Purification of Palmitoyl Protein Thioesterase and Acyl Protein Thioesterase for use in In Vitro Depalmitoylation Reactions
Esoe, Dave-Preston
Erickson, Jeffery A.
Hamann, Michael J.
FASEB JOURNAL, 2019, 33

← 1 2 3 4 5 →