Genetic characterization of Criollo Lageano cattle by RAPD markers

The objective of this study was to characterize genetically the Crioulo Lageano cattle breed, using RAPD markers and compare it to the Holstein and Nelore breeds. Forty three primers were selected, and they generated 77 polymorphic bands. Seven groups were studied: 5 subgroups of Crioulo Lageano (I to V) and one each Holstein (VI) and Nellore (VI 1). Using all groups, the greater part of the genetic variance (65.05%) was due to differences within groups and the rest due to differences between groups. Using five Crioulo Lageano groups (I to V) the results showed 25.28% variation between groups and 74.72% within groups. Genetic diversity has been maintained throughout the generations in this conservation nucleus. The Holstein breed presented the lowest genetic diversity (0.1204) while the Crioulo Lageano herd presented the highest (0.3154). The observed genetic differences were highest between Nellore and Holstein breeds (0.3747), as expected. In general, the Crioulo Lageano groups formed distinct groups and only a few animals from one group were positioned within another group. The RAPD marker technique is adequate to estimate genetic distances between breeds and populations, as well as for use in the choice of individuals for breeding within populations, for conservation of genetic resources.