Biosynthesized Zinc Oxide Nanoparticles Disrupt Established Biofilms of Pathogenic Bacteria

被引:33
|
作者
Husain, Fohad Mabood [1 ]
Qais, Faizan Abul [2 ]
Ahmad, Iqbal [2 ]
Hakeem, Mohammed Jamal [1 ]
Baig, Mohammad Hassan [3 ]
Khan, Javed Masood [1 ]
Al-Shabib, Nasser A. [1 ]
机构
[1] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Riyadh 11451, Saudi Arabia
[2] Aligarh Muslim Univ, Fac Agr Sci, Dept Agr Microbiol, Aligarh 202002, UP, India
[3] Yonsei Univ, Dept Family Med, Coll Med, Gangnam Severance Hosp, 211 Eonju Ro, Seoul 06273, South Korea
来源
APPLIED SCIENCES-BASEL | 2022年 / 12卷 / 02期
关键词
zinc oxide nanoparticles; Plumbago zeylanica; biofilm; EPS; biofilm eradication; GREEN SYNTHESIS; SILVER NANOPARTICLES; LEAF EXTRACT; ANTIBIOFILM; RESISTANCE; L;
D O I
10.3390/app12020710
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Global emergence and persistence of the multidrug-resistant microbes have created a new problem for management of diseases associated with infections. The development of antimicrobial resistance is mainly due to the sub-judicious and unprescribed used of antimicrobials both in healthcare and the environment. Biofilms are important due to their role in microbial infections and hence are considered a novel target in discovery of new antibacterial or antibiofilm agents. In this article, zinc oxide nanoparticles (ZnO-NPs) were prepared using extract of Plumbago zeylanica. ZnO-NPs were characterized and then their antibiofilm activity was tested against Gram-positive and Gram-negative bacteria. The ZnO-NPs were polydispersed, and the average size was obtained as 24.62 nm. The presence of many functional groups indicated that phytocompounds of P. zeylanica were responsible for the synthesis, capping, and stabilization of ZnO-NPs. Synthesized NPs inhibited the biofilm formation of E. coli, S. aureus, and P. aeruginosa by 62.80%, 71.57%, and 77.69%, respectively. Likewise, concentration-dependent inhibition of the EPS production was recorded in all test bacteria. Microscopic examination of the biofilms revealed that ZnO-NPs reduced the bacterial colonization on solid support and altered the architecture of the biofilms. ZnO-NPs also remarkably eradicated the preformed biofilms of the test bacteria up to 52.69%, 59.79%, and 67.22% recorded for E. coli, S. aureus, P. aeruginosa, respectively. The findings reveal the ability of green synthesized zinc oxide nanoparticles to inhibit, as well as eradicate, the biofilms of Gram-positive and Gram-negative bacteria.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Genotoxic Potentials of Biosynthesized Zinc Oxide Nanoparticles
    Gulluce, Medine
    Karadayi, Mehmet
    Demir, Abdussamed Yasin
    Isik, Ceyda
    Alaylar, Burak
    Ispirli, Neslihan Hidiroglu
    POLISH JOURNAL OF ENVIRONMENTAL STUDIES, 2020, 29 (01): : 111 - 119
  • [2] In vitro genotoxicity assessment of biosynthesized zinc oxide nanoparticles
    Demir, Abdussamed Yasin
    Karadayi, Mehmet
    Isaoglu, Mine
    Karadayi, Gokce
    Gulluce, Medine
    TOXICOLOGY AND INDUSTRIAL HEALTH, 2023, 39 (07) : 345 - 355
  • [3] Anticancer Efficacy of Biosynthesized Zinc Oxide and Gold Nanoparticles
    Abdelmonem, M.
    Hammad, S. E.
    Elshikh, H. H.
    Mostafa, M. A.
    EL-Rouby, M. N.
    Wasim, H.
    Abdelmageed, M.
    AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2024, 162 : S121 - S121
  • [4] Bactericidal activity of biosynthesized silver nanoparticles against human pathogenic bacteria
    Abalkhil, Tarad Abdulaziz
    Alharbi, Sulaiman Ali
    Salmen, Saleh Hussein
    Wainwright, Milton
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2017, 31 (02) : 411 - 417
  • [5] Antimicrobial Activities and Mechanisms of Magnesium Oxide Nanoparticles (nMgO) against Pathogenic Bacteria, Yeasts, and Biofilms
    Nhu-Y Thi Nguyen
    Nathaniel Grelling
    Cheyann Lee Wetteland
    Romeo Rosario
    Huinan Liu
    Scientific Reports, 8
  • [6] Antimicrobial Activities and Mechanisms of Magnesium Oxide Nanoparticles (nMgO) against Pathogenic Bacteria, Yeasts, and Biofilms
    Nguyen, Nhu-Y Thi
    Grelling, Nathaniel
    Wetteland, Cheyann Lee
    Rosario, Romeo
    Liu, Huinan
    SCIENTIFIC REPORTS, 2018, 8
  • [7] Biosynthesized Zinc Oxide Nanoparticles as Efficient Photocatalytic and Antimicrobial Agent
    Goyal, Vikas
    Singh, Arashdeep
    Singh, Jagpreet
    Singh, Tarandip
    Al-Kheraif, Abdulaziz A.
    Kaur, Harpreet
    Kumar, Sanjeev
    Rawat, Mohit
    JOURNAL OF CLUSTER SCIENCE, 2022, 33 (06) : 2551 - 2558
  • [8] Kinetics Analysis and Susceptibility Coefficient of the Pathogenic Bacteria by Titanium Dioxide and Zinc Oxide Nanoparticles
    Alizadeh-Sani, Mahmood
    Hamishehkar, Hamed
    Khezerlou, Arezou
    Maleki, Mohammad
    Azizi-Lalabadi, Maryam
    Bagheri, Vahid
    Safaei, Payam
    Azimi, Taher
    Hashemi, Mohammad
    Ehsani, Ali
    ADVANCED PHARMACEUTICAL BULLETIN, 2020, 10 (01) : 56 - 64
  • [9] Rutin-coated zinc oxide nanoparticles: a promising antivirulence formulation against pathogenic bacteria
    Alidoust, Fatemeh Azizi
    Rasti, Behnam
    Zamani, Hojjatolah
    Mirpour, Mirsasan
    Mirzaie, Amir
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2024, 40 (06):
  • [10] In Vitro Evaluation of Antibacterial Properties of Biogenically Synthesized Zinc Oxide Nanoparticles on Pathogenic Paddy Bacteria
    Abd Rahman, Rafidah
    Chia, Chin Hua
    Masdor, Noor Azlina
    JOURNAL OF BIOMIMETICS BIOMATERIALS AND BIOMEDICAL ENGINEERING, 2023, 59 : 1 - 10