Avirulence gene based RFLP and rep-PCR distinguish the genetic variation of Xanthomonas oryzae pv. oryzae pathotypes in Bangladesh

被引:4
|
作者
Haque, Mohammad Mahbubul [1 ]
Masud, Md Mostafa [2 ]
Bashar, Samrin [2 ]
Hossain, Mohammad Iqbal [2 ]
Alam, Md Zahangir [2 ]
Islam, Md Rashidul [2 ]
机构
[1] Bangladesh Inst Nucl Agr BINA, Plant Pathol Div, Mymensingh 2202, Bangladesh
[2] Bangladesh Agr Univ, Dept Plant Pathol, Plant Bacteriol & Biotechnol Lab, Mymensingh 2202, Bangladesh
来源
PLANT SCIENCE TODAY | 2022年 / 9卷 / 01期
关键词
RFLP; Rep-PCR; Pathotypes; BACTERIAL-BLIGHT PATHOGEN; POLYMERASE-CHAIN-REACTION; RESISTANCE GENES; RICE; SEQUENCE; DIVERSITY; PATHOVARS; STRAINS; GENOMES; FINGERPRINT;
D O I
10.14719/pst.1369
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Bacterial blight (BB) caused by X. oryzae pv. oryzae is one of the devastating diseases of rice mostly in Asia. Genomes of X. oryzae pv. oryzae is highly variable due to rearrangement of the large contents of transposable elements and dynamic changes of X. oryzae pv. oryzae population regulated efficiency of the control measures used for BB management of rice worldwide. In this study, genetic variation of X. oryzae pv. oryzae pathotypes of Bangladesh was studied using aviruelnce gene based RFLP and rep-PCR techniques aimed to formulate pathogen targeted effective control measures against BB of rice. Eight pathotypes of X. oryzae pv. oryzae field isolates were identified based on their reactions against 10 Near Isogenic Lines (NILs). Among eight pathotypes, pathotypes IV and V contained higher number of isolates which were 30.13% and 23.01% respectively while pathotype VIII revealed as minimum containing only 2.51% of total isolates. These eight pathotypes were studied for their genetic variation by RFLP using avrBs3 repeat domain as probe. The results conceded that Bangladeshi X. oryzae pv. oryzae strains seem carrying a minimum of two and maximum of nine avrBs3 family genes homologs. The resistance phenotype on IRBB7 and IRBB10 NILs also indicated presence of two major avrBs3 family genes viz. avrxa7 and avrXa10 in some pathotypes. Relationship of phylogenicity exhibited that X. oryzae pv. oryzae pathotypes assorted into two RFLP haplotypes as well as these haplotypes are largely distributed in Bangladesh. Phylogenetic analyses carried out by (REP, ERIC), rep-PCR and BOX depicted the presence of two main molecular haplotypes of X. oryzae pv. oryzae pathotypes. The relationship between pathotypes and molecular haplotypes of X. oryzae pv. oryzae in Bangladesh indicated that the same lineage possesses different pathotypes and different lineage possesses different pathotypes. The results indicated that eight different pathotypes might have originated from common inherited haplotypes with a wide genetic variation.
引用
收藏
页码:29 / 40
页数:12
相关论文
共 50 条
  • [1] Measurement of haplotypic variation in Xanthomonas oryzae within a single field by rep-PCR and RFLP analyses
    Cruz, CMV
    Ardales, EY
    Skinner, DZ
    Talag, J
    Nelson, RJ
    Louws, FJ
    Leung, H
    Mew, TW
    Leach, JE
    PHYTOPATHOLOGY, 1996, 86 (12) : 1352 - 1359
  • [2] Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP
    Zhang, Y.
    Yang, X.
    Zhou, F. Y.
    Zhang, A. F.
    Zhu, X. F.
    Chen, Y.
    Zhou, M. G.
    Gao, T. C.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04) : 18587 - 18595
  • [3] REP-PCR DISTINGUISHES RICE BACTERIAL BLIGHT PATHOGEN (XANTHOMONAS ORYZAE pv. ORYZAE) STRAINS OF INDIAN MAINLAND AND BAY ISLANDS
    Manigundan, K.
    Kumar, P. Puneeth
    Singh, R.
    Sakthivel, K.
    Gautam, R. K.
    Singh, P. K.
    Laha, G. S.
    JOURNAL OF PLANT PATHOLOGY, 2017, 99 (03) : 773 - 778
  • [4] TAL Effectors with Avirulence Activity in African Strains of Xanthomonas oryzae pv. oryzae
    Lachaux, Marlene
    Thomas, Emilie
    Bogdanove, Adam J.
    Szurek, Boris
    Hutin, Mathilde
    RICE, 2022, 15 (01)
  • [5] Genes and proteins involved in aggressiveness and avirulence of Xanthomonas oryzae pv. oryzae to rice
    Leach, JE
    Zhu, W
    Chittoor, JM
    Ponciano, G
    Young, SA
    White, FF
    BIOLOGY OF PLANT-MICROBE INTERACTIONS, 1996, : 191 - 196
  • [6] TAL Effectors with Avirulence Activity in African Strains of Xanthomonas oryzae pv. oryzae
    Marlène Lachaux
    Emilie Thomas
    Adam J. Bogdanove
    Boris Szurek
    Mathilde Hutin
    Rice, 2022, 15
  • [7] Identification of an avirulence gene, avrxa5, from the rice pathogen Xanthomonas oryzae pv. oryzae
    HuaSong Zou
    WenXiang Zhao
    XiFu Zhang
    YangChun Han
    LiFang Zou
    GongYou Chen
    Science China Life Sciences, 2010, 53 : 1440 - 1449
  • [8] Cloning and characterization of a novel avirulence gene (arp3) from Xanthomonas oryzae pv. oryzae
    Liang, B
    Yu, TG
    Guo, B
    Yang, C
    Dai, LY
    Shen, DL
    DNA SEQUENCE, 2004, 15 (02): : 110 - 117
  • [9] Identification of an avirulence gene, avrxa5, from the rice pathogen Xanthomonas oryzae pv. oryzae
    Zou HuaSong
    Zhao WenXiang
    Zhang XiFu
    Han YangChun
    Zou LiFang
    Chen GongYou
    SCIENCE CHINA-LIFE SCIENCES, 2010, 53 (12) : 1440 - 1449
  • [10] Phase variation in rice pathogen Xanthomonas oryzae pv. oryzae
    Madhavan, V. N.
    Patel, H. K.
    Patil, P. B.
    Sonti, R. V.
    MOLECULAR PLANT-MICROBE INTERACTIONS, 2019, 32 (10) : 8 - 8