Sodium chloride-programmed phase transition of β-conglycinin/lysozyme electrostatic complexes from amorphous precipitates to complex coacervates

被引：9

作者：

Zheng, Jiabao ^{[1
]}

Gao, Qing ^{[1
]}

Ge, Ge ^{[1
]}

Wu, Jihong ^{[2
]}

Tang, Chuan-he ^{[1
,3
]}

Zhao, Mouming ^{[1
,2
,3
]}

Sun, Weizheng ^{[1
,3
,4
]}

机构：

[1] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Peoples R China

[2] Beijing Technol & Business Univ BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

[3] Overseas Expertise Intro Ctr Discipline Innovat F, Guangzhou 510641, Peoples R China

[4] Natl Technol Innovat Ctr Synthet Biol, Tianjin 300308, Peoples R China

来源：

FOOD HYDROCOLLOIDS | 2022年 / 124卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Heteroprotein complex coacervation; Liquid-liquid phase separation; Confocal laser scanning microscopy; Isothermal titration calorimetry; Dynamic equilibrium; LYSOZYME; LIQUID;

D O I：

10.1016/j.foodhyd.2021.107247

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The associative phase separation of biomacromolecules can produce both liquid-like coacervates or solid-like precipitates. In this study, we found that sodium chloride (NaCl) can programme the phase transition of beta-conglycinin/lysozyme (beta-CG/LYS) complexes. NaCl reduced the zeta-potential of both beta-CG and LYS. Their complex coacervate was formed at pHs 6 and 7 with 5-80 mM NaCl and at pH 8 with 40-80 mM NaCl. Unlike the high critical salt concentration and entropy gain driving force of polyelectrolyte-based complex coacervation, 100 mM NaCl almost completely inhibited beta-CG/LYS complexation, and the exothermic enthalpy change was the main driving force for beta-CG/LYS complex formation. Confocal laser scanning microscopy with fluorescein isothiocyanate-labeled proteins demonstrated dynamic protein exchange in coacervate droplets, similar to that in polyelectrolyte-based complex coacervates.

引用

页数：10