Identities of two Paragonimus species from Sri Lanka inferred from molecular sequences

被引：14
|
作者：
Iwagami, M
Rajapakse, RPVJ
Paranagama, W
Agatsuma, T [1 ]
机构：
[1] Kochi Med Sch, Dept Environm Hlth Sci, Nankoku, Kochi 7838505, Japan
[2] Univ Peradeniya, Fac Vet Med & Sci, Dept Vet Pathobiol, Peradeniya, Sri Lanka
来源：
JOURNAL OF HELMINTHOLOGY | 2003年 / 77卷 / 03期
关键词：
D O I：
10.1079/JOH2003180
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Metacercariae of Paragonimus spp. were obtained from field-collected freshwater crabs in Sri Lanka. Genomic DNA was extracted from single metacercariae. Two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified using the polymerase chain reaction. Two differing sequences were obtained for each of these gene regions. Phylogenetic analyses placed the type 1 sequences as sister to a clade containing P. westermani and P. siamensis whereas the type 2 sequences were close to published sequences of P. siamensis from Thailand. The possible taxonomic status of these two types are discussed. This is the first report of molecular data about Paragonimus from Sri Lanka.
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页码：239 / 245
页数：7
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