Use of the affinity chromatography principle in creating new thromboresistant materials

The principle of affinity chromatography was used for preparation of thromboresistant bilayer coatings. The outer biospecific layer containing E-aminocaproic acid residues (from 2.2 up to 5.5 nmol/cm(2)) was synthesized using a copolymer of maleic anhydride with N-vinylpyrrolidone and L-lysine dihydrochloride or N-epsilon-tert-BOC-L-lysine. This surface can selectively adsorb plasminogen (fibrinolytic zymogen) from blood. The biospecific layer (from 2.0 up to 3.6 mug/cm(2)) was applied for covering chitosan (native or modified) or albumin interlayer. Such bilayer coatings (BCs) were stable and represented the insoluble polyelectrolyte complexes. BCs were proposed for bilayer modification of synthetic vascular grafts, polyethylene, and other materials contacting with blood. This technique allowed us to significantly reduce thrombogenic properties of polyethylene surfaces. (C) 2003 Elsevier B.V. All rights reserved.