Isolation and characterization of microsatellite loci in two species-at-risk in British Columbia: Great Basin spadefoot (Spea intermontana) and Western painted turtle (Chrysemys picta bellii)

被引：0

作者：

de Silva, Anders Goncalves ^{[1
,2
]}

Williams, Kathleen E. ^{[1
,2
]}

Kirk, Stephanie L. ^{[1
,2
]}

Bishop, Christine A. ^{[3
]}

Hodges, Karen E. ^{[1
,2
]}

Russello, Michael A. ^{[1
,2
]}

机构：

[1] Univ British Columbia Okanagan, Dept Biol, Kelowna, BC V1V 1V7, Canada

[2] Univ British Columbia Okanagan, Ctr Species Risk & Habitat Studies, Kelowna, BC V1V 1V7, Canada

[3] Environm Canada, Sci & Technol Branch, Delta, BC, Canada

来源：

CONSERVATION GENETICS RESOURCES | 2010年 / 2卷 / 01期

关键词：

Amphibians; Dinucleotides; Microsatellites; Polymorphic; Primers; Reptiles; Tetranucleotides;

D O I：

10.1007/s12686-009-9136-2

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Seventeen polymorphic microsatellite loci were characterized in two Canadian species-at-risk, Great Basin spadefoot (Spea intermontana, N = 9) and the Western painted turtle (Chrysemys picta bellii, N = 8), from GT(n) enriched genomic libraries and cross-taxa amplification. Number of alleles per locus ranged from 2 to 9, with an average of 3.9 and 4.3 alleles/locus in Great Basin spadefoot and Western painted turtle, respectively. Mean expected heterozygosities were comparatively high (0.43-0.52), as was the power to distinguish between individuals relative to the number of loci characterized per species (multilocus P-ID = 9.2 x 10(-5) -1.6 x 10(-5)). These loci will be fundamental in generating historical population information in support of conservation efforts for these two imperiled species.

引用

页码：37 / 40

页数：4