Marinobacter salexigens sp nov., isolated from marine sediment

A novel bacterium, designated as strain HJR7(T), was isolated from a marine sediment sample collected from the coastal area of Weihai, China (121 degrees 57 ' E, 37 degrees 29 ' N). Cells were Gram-stain-negative, facultative anaerobic, non-motile and rod-shaped. The temperature, pH and NaCl ranges for growth were determined as 4-40 degrees C, pH 6.5-9.5 and 0.5-15.0 % (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain HJR7(T) belongs to the genus Marinobacter in the family Alteromonadaceae. The most closely related species were Marinobacter aromaticivorans (97.6% 16S rRNA gene sequence similarity) and Marinobacter maritimus (97.3% similarity). Ubiquinone 9 (Q-9) was the only respiratory quinone detected in strain HJR7(T). The major fatty acids of strain HJR7(T) were C-12:0,C- C-16:0,C- C-16:0 N alcohol, C-18:1 omega 9c and C-18:3 omega 6, 9, 12c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The DNA G+C content of strain HJR7(T) was 53.7 mol%. On the basis of phylogenetic, genotypic, phenotypic, and chemotaxonomic analyses, strain HJR7(T) represents a novel species within the genus Marinobacter, for which the name Marinobacter salexigens sp. nov. is proposed. The type strain is HJR7(T) (= KCTC 52545(T) =MCCC 1H00176(T)).