Diffusion tensor imaging of human Achilles tendon by stimulated echo readout-segmented EPI (ste-RS-EPI)

Purpose: Healing, regeneration, and remodeling of the injured Achilles tendon are associated with notable changes in tendon architecture. However, assessing Achilles microstructural properties with conventional diffusion tension imaging (DTI) remains a challenge because of very short T-2/T-2(chi) values of the tendon. Hence, the objective of this study was to develop a novel Achilles tendon DTI protocol for a non-invasive investigation of the changes of microstructural integrity in tendinopathy. Methods: A novel stimulated echo readout-segmented EPI (ste-RS-EPI) DTI sequence was proposed to achieve a TE of similar to 14-20 ms for typical b-values of 400-800 s/mm(2) on clinical 3T MRI scanners. To further boost tendon MR signal, the Achilles was positioned at the magic angle (similar to 55 degrees) with respect to the scanner B-0 field. The sensitivity of the developed protocol was evaluated in 19 healthy participants and 6 patients with clinically confirmed tendinopathy. Results: Compared to spin echo RS-EPI DTI protocol, ste-RS-EPI provided an similar to 100-200% increase in Achilles MR signal. Tendinopathic Achilles demonstrated a high degree of microstructural disruption based on DTI tractography analysis, with significantly lower (P<0.05) axial diffusivity (1.20 +/- 0.19 vs. 1.39 +/- 0.10x10(-3) mm(2)/s), radial diffusivity (0.72 +/- 0.11 vs. 0.81 +/- 0.08x10(-3) mm(2)/s), and mean diffusivity (0.87 +/- 0.14 vs. 1.00 +/- 0.07x10(-3) mm(2)/s), but no significant difference in fractional anisotropy (0.38 +/- 0.04 vs. 0.38 +/- 0.05; P=0.86). Conclusion: Achilles tendon ste-RS-EPI DTI can non-invasively detect the tendinopathy-induced changes to microstructural integrity, consistent with the disruption of collagen arrangement and increased cellularity. This study demonstrated the robustness and sensitivity of the proposed protocol in Achilles tendinopathy.