Batch and fed-batch fermentation of Bacillus thuringiensis using starch industry wastewater as fermentation substrate

被引：9

作者：

Vu, Khanh Dang ^{[1
]}

Tyagi, Rajeshwar Dayal ^{[1
]}

Valero, Jose R. ^{[1
]}

Surampalli, Rao Y. ^{[2
]}

机构：

[1] Univ Quebec, INRS ETE, Quebec City, PQ G1K 9A9, Canada

[2] US EPA, Kansas City, KS 66117 USA

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2010年 / 33卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Bacillus thuringiensis; Starch industry wastewater; Fed-batch culture; Entomotoxicity; Delta-endotoxin; INSECTICIDAL CRYSTAL PROTEIN; DELTA-ENDOTOXIN PRODUCTION; CONTINUOUS-CULTURE; ZWITTERMICIN-A; SUBSP KURSTAKI; GROWTH; BIOPESTICIDES; SPORULATION; CEREUS; MEDIA;

D O I：

10.1007/s00449-009-0391-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus thuringiensis var. kurstaki biopesticide was produced in batch and fed-batch fermentation modes using starch industry wastewater as sole substrate. Fed-batch fermentation with two intermittent feeds (at 10 and 20 h) during the fermentation of 72 h gave the maximum delta-endotoxin concentration (1,672.6 mg/L) and entomotoxicity (Tx) (18.5 x 10(6) SBU/mL) in fermented broth which were significantly higher than maximum delta-endotoxin concentration (511.0 mg/L) and Tx (15.8 x 10(6) SBU/mL) obtained in batch process. However, fed-batch fermentation with three intermittent feeds (at 10, 20 and 34 h) of the fermentation resulted in the formation of asporogenous variant (Spo-) from 36 h to the end of fermentation (72 h) which resulted in a significant decrease in spore and delta-endotoxin concentration and finally the Tx value. Tx of suspended pellets (27.4 x 10(6) SBU/mL) obtained in fed-batch fermentation with two feeds was the highest value as compared to other cases.

引用

页码：691 / 700

页数：10

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