Antimutagenicity of hops (Humulus lupulus L.):: bioassay-directed fractionation and isolation of xanthohumol

被引：24

作者：

Kac, Javor ^{[2
]}

Plazar, Janja ^{[1
]}

Mlinaric, Ales ^{[2
]}

Zegura, Bojana ^{[1
]}

Lah, Tamara T. ^{[1
]}

Filipic, Metka ^{[1
]}

机构：

[1] Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana 1000, Slovenia

[2] Univ Ljubljana, Fac Pharm, Dept Pharmaceut Biol, Ljubljana 1000, Slovenia

来源：

PHYTOMEDICINE | 2008年 / 15卷 / 03期

关键词：

antimutagen; comet assay; HepG2; Humulus lupulus; Salmonella/microsomal assay; xanthohumol;

D O I：

10.1016/j.phymed.2007.09.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bioassay-directed fractionation with a Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5-f]quinohne (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by IQ. Fractionation of the diethylether extract (DE) by column chromatography, followed by semi-preparative HPLC yielded two fractions (E4b and E4d) with strong antimutagenic activity against IQ induced mutations. Separation of fraction E4b resulted in inactive fractions, while fraction E4d has been identified to be xanthohumol. In mammalian test system with human hepatoma HepG2 cells fraction E4d at 10 mu g/ml completely prevented fori-nation of IQ induced DNA damage. These results indicate that xanthohumol is a very promising potential protective agent against genotoxicity of food borne carcinogens, which warrants further investigation. (c) 2007 Elsevier GmbH. All rights reserved.

引用

页码：216 / 220

页数：5

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