Elucidation of the Potential Hair Growth-Promoting Effect of Botryococcus terribilis, Its Novel Compound Methylated-Meijicoccene, and C32 Botryococcene on Cultured Hair Follicle Dermal Papilla Cells Using DNA Microarray Gene Expression Analysis

被引：7

作者：

Oliva, Aprill Kee ^{[1
,2
]}

Bejaoui, Meriem ^{[1
,2
,3
]}

Hirano, Atsushi

Arimura, Takashi ^{[3
,4
]}

Linh, Tran Ngoc ^{[3
]}

Uchiage, Eriko ^{[3
]}

Nukaga, Sachiko ^{[4
]}

Tominaga, Kenichi ^{[3
]}

Nozaki, Hiroyuki ^{[4
]}

Isoda, Hiroko ^{[1
,2
,3
,5
]}

机构：

[1] Univ Tsukuba, Res & & Dev Ctr, Tailor Made QOL Program, Tsukuba, Ibaraki 3058550, Japan

[2] Univ Tsukuba, Alliance Res Mediterranean & North Africa ARENA, Tsukuba, Ibaraki 3058572, Japan

[3] Univ Tsukuba, Open Innovat Lab Food & Med Resource Engn FoodMed, AIST, Tsukuba, Ibaraki 3050006, Japan

[4] Tokyo Elect Power Co Holdings Inc, Chiyoda Ku, 1-1-3 Uchisaiwai Cho, Tokyo 1008560, Japan

[5] Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

来源：

BIOMEDICINES | 2022年 / 10卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

Botryococcus terribilis; me-meijicoccene; C32; botryococcene; dermal papilla cells; hair growth; STEM-CELLS; MECHANISMS; DIFFERENTIATION; PROLIFERATION; MORPHOGENESIS; CHEMISTRY; EXTRACT; RACE; SKIN;

D O I：

10.3390/biomedicines10051186

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A person's quality of life can be adversely affected by hair loss. Microalgae are widely recognized for their abundance and rich functional components. Here, we evaluated the hair growth effect of a green alga, Botryococcus terribilis (B. terribilis), in vitro using hair follicle dermal papilla cells (HFDPCs). We isolated two types of cells from B. terribilis - green and orange cells, obtained from two different culture conditions. Microarray and real time-PCR results revealed that both cell types stimulated the expression of several pathways and genes associated with different aspect of the hair follicle cycle. Additionally, we demonstrated B. terribilis' effect on collagen and keratin synthesis and inflammation reduction. We successfully isolated a novel compound, methylated-meijicoccene (me-meijicoccene), and C32 botryococcene from B. terribilis to validate their promising effects. Our study revealed that treatment with the two compounds had no cytotoxic effect on HFDPCs and significantly enhanced the gene expression levels of hair growth markers at low concentrations. Our study provides the first evidence of the underlying hair growth promoting effect of B. terribilis and its novel compound, me-meijicoccene, and C32 botryococcene.

引用

页数：21