Use of PCR amplification of tRNA gene-linked short tandem repeats for genotyping Entamoeba histolytica

被引：77

作者：

Ali, IKM ^{[1
]}

Zaki, M ^{[1
]}

Clark, CG ^{[1
]}

机构：

[1] Univ London London Sch Hyg & Trop Med, Dept Infect Dis & Trop Dis, London WC1E 7HT, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 12期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JCM.43.12.5842-5847.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have developed a reliable method for PCR-based genotyping of Entamoeba histolytica based on variation in the numbers of short tandem repeats that are linked to tRNA genes in this species. Species-specific primer pairs were designed that differentiate E. histolytica from E. dispar as well as that reveal intraspecies PCR product length polymorphisms. The primers were tested with samples from different parts of the world, and DNA was extracted from cultured cells as well as liver abscess pus and feces by various methods. We now have the tools necessary to investigate a possible link between parasite genotype and the outcome of infection with Entamoeba histolytica, as well as other aspects of the organism's epidemiology.

引用

页码：5842 / 5847

页数：6

共 15 条

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[8] Use of X-linked short tandem repeats loci to confirm mutations in parentage caseworks
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