Glycosylphosphatidylinositol-anchored fungal polysaccharide in Aspergillus fumigatus

被引:80
|
作者
Costachel, C
Coddeville, B
Latgé, JP
Fontaine, T
机构
[1] Inst Pasteur, Unite Aspergillus, F-75724 Paris, France
[2] Univ Sci & Technol Lille, Chim Biol Lab, F-59655 Villeneuve Dascq, France
关键词
D O I
10.1074/jbc.M510163200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Galactomannan is a characteristic polysaccharide of the human filamentous fungal pathogen Aspergillus fumigatus that can be used to diagnose invasive aspergillosis. In this study, we report the isolation of a galactomannan fraction associated to membrane preparations from A. fumigatus mycelium by a lipid anchor. Specific chemical and enzymatic degradations and mass spectrometry analysis showed that the lipid anchor is a glycosylphosphatidylinositol ( GPI). The lipid part is an inositol phosphoceramide containing mainly C-18-phytosphingosine and monohydroxylated lignoceric acid ( 20H-C-24:0 fatty acid). GPI glycan is a tetramannose structure linked to a glucosamine residue: Man alpha 1-2Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN. The galactomannan polymer is linked to the GPI structure throught the mannan chain. The GPI structure is a type 1, closely related to the one previously described for the GPI-anchored proteins of A. fumigatus. This is the first time that a fungal polysaccharide is shown to be GPI-anchored.
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收藏
页码:39835 / 39842
页数:8
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