Recovery of DNA from agarose gel by trap method

被引：0

作者：

Xia, Hongli ^{[1
]}

Tan, Zui ^{[1
]}

Qiao, Jianguo ^{[2
]}

Liang, Chuan ^{[1
]}

机构：

[1] Wuhan Univ, Dept Vasc Surg, Zhongnan Hosp, Wuhan 430071, Peoples R China

[2] Wuhan Univ, Dept Gen Surg, Zhongnan Hosp, Wuhan 430071, Peoples R China

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 50期

基金：

中国国家自然科学基金;

关键词：

Trap method; recovery of DNA; agarose gel electrophoresis; FREE SOLUTION MOBILITY; PHYSICAL-PROPERTIES; ELECTROPHORESIS; BUFFERS; EFFICIENT; FRAGMENTS;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recovery of DNA from agarose gel electrophoresis is a basic operation during molecular cloning. Circular or linear DNA fragments which vary from 1.5 to 6.5 kb and correspond to 1 kb marker can be recovered from 0.8 to 1.0% agarose gel smoothly with a simple and rapid trap method. The recovery efficiency could be more than 70% and the quality of the recovered DNA is proved to be good enough for future research. Here, we provided another good method to recover DNA from agarose gel besides using commercial kits.

引用

页码：10280 / 10286

页数：7

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