Optical assessment of Dunaliella salina microalgae viability upon toxicological testing

被引：1
|
作者：
Chumakov, D. S. [1 ]
Golubev, A. A. [1 ]
Dykman, L. A. [1 ]
Khlebtsov, N. G. [1 ]
Bogatyrev, V. A. [1 ]
机构：
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia
来源：
COLLOID JOURNAL | 2017年 / 79卷 / 06期
基金：
俄罗斯基础研究基金会;
关键词：
PARTICLE-SIZE; GOLD; SUSPENSIONS; ABSORPTION; CLUSTERS;
D O I：
10.1134/S1061933X17060059
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of an absorption peak at 680 nm corrected for the nonselective extinction as calculated using the values of nearest local minima at 640 and 740 nm.
引用
收藏
页码：844 / 848
页数：5
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