Functional Expression of phaCAB Genes from Cupriavidus taiwanensis Strain 184 in Escherichia coli for Polyhydroxybutyrate Production

被引：13
|
作者：
Chien, Chih-Ching [1 ]
Hong, Chian-Chiao [1 ]
Soo, Po-Chi [3 ]
Wei, Yu-Hong [1 ]
Chen, Shan-Yu [1 ]
Cheng, Mei-Ling [2 ]
Sun, Yi-Ming [2 ]
机构：
[1] Yuan Ze Univ, Grad Sch Biotechnol & Bioengn, Chungli 320, Taiwan
[2] Yuan Ze Univ, Dept Chem Engn & Mat Sci, Chungli 320, Taiwan
[3] Tzu Chi Univ, Dept Lab Med & Biotechnol, Hualien 970, Taiwan
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 162卷 / 08期
关键词：
Polyhydroxyalkanoates; PHA; Cupriavidus taiwanensis; PHB; Polyhydroxybutyrate; POLY-BETA-HYDROXYBUTYRATE; POLYHYDROXYALKANOATES; BIOSYNTHESIS; POLYESTER; BACTERIA; ACID; PHB;
D O I：
10.1007/s12010-010-9008-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polyhydroxyalkanoates are polyesters synthesized by numerous microorganisms. These polyesters are biodegradable and have similar properties to those of conventional plastics. Cupriavidus taiwanensis strain 184 is phylogenetically related to the well-known polyhydroxybutyrate (PHB) producer Ralstonia eutropha (Cupriavidus necator) and is also shown to be able to accumulate significant amounts of PHB. In this study, we cloned the PHB synthesis genes (phaCAB) from C. taiwanensis 184 into Escherichia coli for biosynthesis of PHB. The recombinant E. coli strains were able to synthesize significant amounts of PHB. The PHB amounted to about 66 similar to 70% of total cell material of these recombinant strains.
引用
收藏
页码：2355 / 2364
页数：10
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