Carboxy-terminal modification of polyhydroxyalkanoate (PHA) via alcoholysis reaction catalyzed by Class IV PHA synthase

被引：15
|
作者：
Hyakutake, Manami [1 ,2 ]
Tomizawa, Satoshi [3 ]
Sugahara, Imari [1 ]
Murata, Erni [1 ]
Mizuno, Kouhei [4 ]
Abe, Hideki [1 ,2 ]
Tsuge, Takeharu [1 ]
机构：
[1] Tokyo Inst Technol, Dept Innovat & Engn Mat, Midori Ku, Yokohama, Kanagawa 2268502, Japan
[2] RIKEN, Ctr Sustainable Resource Sci, Biomass Engn Res Div, Wako, Saitama 3510198, Japan
[3] Kumamoto Natl Coll Technol, Dept Biol & Chem Syst Engn, Yatsushiro, Kumamoto 8668501, Japan
[4] Kitakyushu Natl Coll Technol, Dept Mat Sci & Chem Engn, Kokuraminami Ku, Kitakyushu, Fukuoka 8020985, Japan
来源：
POLYMER DEGRADATION AND STABILITY | 2015年 / 117卷
关键词：
Alcoholytic cleavage; Polyhydroxyalkanoate synthase; Bacillus; Carboxy-terminal modification; Functionalization; RECOMBINANT ESCHERICHIA-COLI; CHAIN TRANSFER AGENTS; BACILLUS-CEREUS YB-4; MOLECULAR-WEIGHT; RALSTONIA-EUTROPHA; BIOSYNTHESIS; GLYCEROL; ETHANOL; BLENDS; STRAIN;
D O I：
10.1016/j.polymdegradstab.2015.04.002
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Polyhydroxyalkanoate (PHA) synthase from the bacterium Bacillus cereus YB-4 (PhaRC(YB4)), a class IV PHA synthase, shows both PHA polymerization and alcoholysis activities. In PhaRC(YB4)-expressing Escherichia coli in the presence of ethanol, biosynthesized PHA is subjected to an ethyl-esterification at the carboxy terminus as a result of alcoholytic cleavage of the PHA chain by PhaRC(YB4). In this study, we investigated the range of alcohol species available for alcoholysis by PhaRC(YB4) using E. coli JM109 as an assay host by monitoring the molecular weight of PHA during the second step of a two-step cultivation. PhaRC(YB4) utilized various alcohols other than ethanol for alcoholysis. Through the use of bifunctional compounds for alcoholysis, the PHA carboxy terminus could be modified with thiol, alkynyl, hydroxy, and benzyl groups, resulting in the functionalization of the PHA carboxy terminus. Furthermore, one-step production of carboxy-terminal functionalized PHA was developed using E. coli XL1-Blue as a production host. (c) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：90 / 96
页数：7
相关论文
共 17 条
  • [1] Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus
    Takeharu Tsuge
    Manami Hyakutake
    Kouhei Mizuno
    [J]. Applied Microbiology and Biotechnology, 2015, 99 : 6231 - 6240
  • [2] Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus
    Tsuge, Takeharu
    Hyakutake, Manami
    Mizuno, Kouhei
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 99 (15) : 6231 - 6240
  • [3] Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase
    Tian, J
    Sinskey, AJ
    Stubbe, J
    [J]. BIOCHEMISTRY, 2005, 44 (05) : 1495 - 1503
  • [4] Polyhydroxyalkanoate (PHA) accumulation in sulfate-reducing bacteria and identification of a class III PHA synthase (PhaEC) in Desulfococcus multivorans
    Hai, T
    Lange, D
    Rabus, R
    Steinbüchel, A
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (08) : 4440 - 4448
  • [5] Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB-4 as Host Strain
    Hyakutake, Manami
    Saito, Yuta
    Tomizawa, Satoshi
    Mizuno, Kouhei
    Tsuge, Takeharu
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2011, 75 (08) : 1615 - 1617
  • [6] Location of functional region at N-terminus of polyhydroxyalkanoate (PHA) synthase by N-terminal mutation and its effects on PHA synthesis
    Ye, Ziqiang
    Song, Ge
    Chen, Guoqiang
    Chen, Jingyu
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2008, 41 (01) : 67 - 73
  • [7] In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: a useful technique for surface coatings of a hydrophobic support with PHA
    Sato, Shun
    Minato, Masayuki
    Kikkawa, Yoshihiro
    Abe, Hideki
    Tsuge, Takeharu
    [J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2010, 85 (06) : 779 - 782
  • [8] Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate
    Chen, Yi-Jr
    Tsai, Pei-Chien
    Hsu, Chun-Hua
    Lee, Chia-Yin
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 2014, 56 : 60 - 66
  • [9] Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli
    Shi, Mengxun
    Li, Mengdi
    Yang, Anran
    Miao, Xue
    Yang, Liu
    Pandhal, Jagroop
    Zou, Huibin
    [J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
  • [10] Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers
    Shen, Xiao-Wen
    Shi, Zhen-Yu
    Song, Ge
    Li, Zheng-Jun
    Chen, Guo-Qiang
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 91 (03) : 655 - 665
12