Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3)

被引：0
|
作者：
Hanapiah, Rafidah Mohamed Ali [1 ]
Abu Samah, Rozaimi [1 ]
Rodzri, Nurul Atikah Mohd [1 ]
Zahari, Mior Ahmad Khushairi Mohd [1 ]
Zain, Wan Salwanis Wan Md [1 ]
机构：
[1] Univ Malaysia Pahang, Fac Chem & Nat Resources Engn, Kuantan 26300, Pahang, Malaysia
来源：
MATERIALS TODAY-PROCEEDINGS | 2019年 / 19卷
关键词：
Xylonic acid; Recombinant Escherichia coli; Xylose; SOB Medium; D-XYLOSE;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 degrees C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract. (C) 2019 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1467 / 1472
页数：6
相关论文
共 50 条
  • [1] Effect of Physical Parameters on Production of D-Xylonic Acid using Recombinant E. coli BL21 (DE3)
    Rodzri, N. A. M.
    Zain, W. S. W. M.
    Hanapiah, R. M. A.
    Samah, R. A.
    Illias, R. M.
    [J]. MATERIALS TODAY-PROCEEDINGS, 2019, 19 : 1247 - 1254
  • [2] On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains
    Carmignotto, Gabriela Pannunzio
    Azzoni, Adriano Rodrigues
    [J]. JOURNAL OF BIOTECHNOLOGY, 2019, 306 : 62 - 70
  • [3] E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)
    Johanna Hausjell
    Julia Weissensteiner
    Christian Molitor
    Heidi Halbwirth
    Oliver Spadiut
    [J]. Microbial Cell Factories, 17
  • [4] Influence of the Operating Parameters on the E. Coli BL21 (DE3) Growth in Fedbatch Bioreactor
    Isopencu, Gabriela
    Tanase, Alina
    Josceanu, Anamaria
    Lavric, Vasile
    [J]. REVISTA DE CHIMIE, 2014, 65 (12): : 1511 - 1516
  • [5] Cloning and expression of staphylokinase-streptokinase recombinant protein in E. coli BL21(DE3)
    Harith K. Buniya
    Almuthana K. Hameed
    Anas Y. Al-Hayawi
    [J]. Biologia, 2023, 78 : 1113 - 1117
  • [6] Cloning and expression of staphylokinase-streptokinase recombinant protein in E. coli BL21(DE3)
    Buniya, Harith K. K.
    Hameed, Almuthana K. K.
    Al-Hayawi, Anas Y. Y.
    [J]. BIOLOGIA, 2023, 78 (04) : 1113 - 1117
  • [7] Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
    Stefan Kittler
    Christoph Slouka
    Andreas Pell
    Roman Lamplot
    Mihail Besleaga
    Sarah Ablasser
    Christoph Herwig
    Oliver Spadiut
    Julian Kopp
    [J]. Scientific Reports, 11
  • [8] Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
    Kittler, Stefan
    Slouka, Christoph
    Pell, Andreas
    Lamplot, Roman
    Besleaga, Mihail
    Ablasser, Sarah
    Herwig, Christoph
    Spadiut, Oliver
    Kopp, Julian
    [J]. SCIENTIFIC REPORTS, 2021, 11 (01)
  • [9] Overexpression of a lethal methylase, M.TneDI, in E. coli BL21(DE3)
    Hui Xu
    Dongmei Han
    Zhaohui Xu
    [J]. Biotechnology Letters, 2014, 36 : 1853 - 1859
  • [10] The expression analysis of recombinant protein in Escherichia coli BL21 (DE3)
    [J]. 2001, Beijing Forestry University (23):
12345