Immobilization of Mucor java']javanicus lipase by entrapping in alginate-silica hybrid gel beads with simultaneous cross-linking with glutaraldehyde

被引：11

作者：

Wu, Jin Chuan ^{[1
]}

Wong, Yew Kong ^{[1
]}

Chang, Kiat Wei ^{[1
]}

Tay, Chor Yong ^{[1
]}

Chow, Yvonne ^{[1
]}

Talukder, M. M. R. ^{[1
]}

Choi, Won Jae ^{[1
]}

机构：

[1] Inst Chem & Engn Sci, Singapore 627833, Singapore

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2007年 / 25卷 / 06期

关键词：

alginate; cross-linking; entrapment; hybrid gel; lipase; Mucor [!text type='java']java[!/text]nicus; silica;

D O I：

10.1080/10242420701661149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mucor javanicus lipase was entrapped in alginate-silica hybrid gel beads with or without simultaneous cross-linking with glutaraldehyde. The activity and recovery of activity on immobilization of the enzyme entrapped in the hybrid beads were 1.4 and 1.7 times higher than those of the enzyme entrapped in the simple alginate beads. Entrapment with simultaneous cross-linking in the hybrid beads further improved the enzyme activity (1.6 times) and activity recovery ( 1.7 times) compared to those of the enzyme entrapped in the hybrid beads without simultaneous cross-linking. The leakage of the enzyme entrapped in the hybrid beads with simultaneous cross-linking was only 50% that of the enzyme entrapped in the simple alginate beads.

引用

页码：459 / 463

页数：5

共 11 条

[1] Immobilization of Candida rugosa lipase by cross-linking with glutaraldehyde followed by entrapment in alginate beads
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