Real-time monitoring of the aggregation of Alzheimer's amyloid-β via 1H magic angle spinning NMR spectroscopy

被引：32

作者：

Wang, Jian ^{[1
,2
]}

Yamamoto, Tomoya ^{[1
,2
]}

Bai, Jia ^{[1
,2
]}

Cox, Sarah J. ^{[2
]}

Korshavn, Kyle J. ^{[1
,2
]}

Monette, Martine ^{[3
]}

Ramamoorthy, Ayyalusamy ^{[1
,2
]}

机构：

[1] Univ Michigan, Biophys Program, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[3] Bruker Canada Ltd, 2800 High Point Dr, Milton, ON L9T 5G5, Canada

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 16期

关键词：

DISEASE; PROTEIN; OLIGOMERS; KINETICS; GROWTH; RESOLUTION; MECHANISM; TOXICITY; INSIGHTS; FIBRILS;

D O I：

10.1039/c8cc00167g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Proton magic-angle-spinning NMR used for real-time analysis of amyloid aggregation reveals that mechanical rotation of A beta(1-40) monomers increases the rate of formation of aggregates, and that the increasing lag-time with peptide concentration suggests the formation of growth-incompetent species. EGCG's ability to shift off-pathway aggregation is also demonstrated.

引用

页码：2000 / 2003

页数：4

共 50 条

[1] Monitoring apoptosis in intact cells by high-resolution magic angle spinning 1H NMR spectroscopy
Wylot, Marta
Whittaker, David T. E.
Wren, Stephen A. C.
Bothwell, John H.
Hughes, Leslie
Griffin, Julian L.
[J]. NMR IN BIOMEDICINE, 2021, 34 (03)
[2] Origins of linewidth in 1H magic-angle spinning NMR
Zorin, Vadim E.
Brown, Steven P.
Hodgkinson, Paul
[J]. JOURNAL OF CHEMICAL PHYSICS, 2006, 125 (14):
[3] Real-Time Monitoring of Alzheimer's-Related Amyloid Aggregation via Probe Enhancement-Fluorescence Correlation Spectroscopy
Guan, Yinghua
Cao, Kevin J.
Cantlon, Adam
Elbel, Kristyna
Theodorakis, Emmanuel A.
Walsh, Dominic M.
Yang, Jerry
Shah, Jagesh V.
[J]. ACS CHEMICAL NEUROSCIENCE, 2015, 6 (09): : 1503 - 1508
[4] The biochemical profile of rat testicular tissue as measured by magic angle spinning 1H NMR spectroscopy
Griffin, JL
Troke, J
Walker, LA
Shore, RF
Lindon, JC
Nicholson, JK
[J]. FEBS LETTERS, 2000, 486 (03) : 225 - 229
[5] Use of Magic Angle Spinning 1H NMR Spectroscopy to Monitor Reactions in Solid-Phase Synthesis
Ai Ming YU
Hua Zheng YANG(The State Key Laboratory of Elemento-Organic Chemistry
Zhen Pu ZHANG
Ming LI(The State Key Laboratory of Functional Polymeric Materials for Adsorption and Separation
[J]. Chinese Chemical Letters, 1997, (12) : 0 - 0
[6] Forensic examination of electrical tapes using high resolution magic angle spinning 1H NMR spectroscopy
Schoenberger, Torsten
Simmross, Ulrich
Poppe, Christian
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (01) : 123 - 129
[7] Use of magic angle spinning 1H NMR spectroscopy to monitor reactions in solid-phase synthesis
Yu, AM
Yang, HZ
Sun, PC
Zhang, ZP
Li, M
[J]. CHINESE CHEMICAL LETTERS, 1997, 8 (12) : 1063 - 1064
[8] Forensic examination of electrical tapes using high resolution magic angle spinning 1H NMR spectroscopy
Torsten Schoenberger
Ulrich Simmross
Christian Poppe
[J]. Analytical and Bioanalytical Chemistry, 2016, 408 : 123 - 129
[9] High-resolution 1H NMR spectroscopy in organs and tissues using slow magic angle spinning
Wind, RA
Hu, JZ
Rommereim, DN
[J]. MAGNETIC RESONANCE IN MEDICINE, 2001, 46 (02) : 213 - 218
[10] High-resolution 1H and 1H-13C magic angle spinning NMR spectroscopy of rat liver
Bollard, ME
Garrod, S
Holmes, E
Lincoln, JC
Humpfer, E
Spraul, M
Nicholson, JK
[J]. MAGNETIC RESONANCE IN MEDICINE, 2000, 44 (02) : 201 - 207

← 1 2 3 4 5 →