Synthesis of disaccharide derivatives employing β-N-acetyl-D-hexosaminidase, β-D-galactosidase and β-D-glucuronidase

被引：8
|
作者：
Nilsson, KGI [1 ]
Eliasson, A [1 ]
Pan, HF [1 ]
Rohman, M [1 ]
机构：
[1] Glycorex AB, S-22370 Lund, Sweden
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 01期
关键词：
glycosidase; disaccharide; derivative; beta-D-glucuronidase; beta-N-acetyl-D-hexosaminidase;
D O I：
10.1023/A:1005439302682
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-N-Acetyl-D-hexosaminidase from Aspergillus oryzae catalysed the stereo- and regiospecific formation of the 6-O-benzylated disaccharide derivatives GalNAc beta 1-3(6-OBn)Gal beta-SEt and GlcNAc beta l-3(6-OBn)Gal beta-SEt, which were obtained in transglycosylation reactions employing ethyl 6-O-benzyl-1-thio-beta-D-galactopyranoside as acceptor. Preparative amounts of the chitobiose derivative GlcNAc beta 1-3GlcNAc beta-OPhNO2-p was prepared as well. beta-N-Acetyl-D-hexosaminidase from bovine testes catalysed the specific synthesis of GlcNAc beta 1-3(6-OBn)GlcNH(2)beta-SEt and GalNAc beta 1-3(6-OBn)GlcNH(2)beta-SEt, employing ethyl 2-amino-6-O-benzyl-2-deoxy-1-thio-beta-D-glucopyranoside as acceptor. B-D-Glucuronidase from E. coli was found to catalyse the formation of GlcA beta 1-3(6-OBn)GlcNH(2)beta-SEt employing the same acceptor.
引用
收藏
页码：11 / 15
页数：5
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