Dicentric assay: Inter-laboratory comparison in Indian laboratories for routine and triage applications

被引：18
|
作者：
Bakkiam, D. [1 ]
Bhavani, M. [2 ]
Kumar, A. Arul Anantha [1 ]
Sonwani, Swetha [1 ]
Venkatachalam, P. [2 ]
Sivasubramanian, K. [1 ]
Venkatraman, B. [1 ]
机构：
[1] Indira Gandhi Ctr Atom Res, Radiol Safety Div, Kalpakkam 603102, Tamil Nadu, India
[2] Sri Ramachandra Univ, Madras 600116, Tamil Nadu, India
来源：
APPLIED RADIATION AND ISOTOPES | 2015年 / 99卷
关键词：
Biodosimetry; Dicentric assay; Inter-laboratory comparison; Triage dose estimation; BIOLOGICAL DOSIMETRY; CHROMOSOME ASSAY; CYTOGENETIC DOSIMETRY; RADIATION; BIODOSIMETRY; CASUALTIES; OVEREXPOSURE; ACCIDENTS; NETWORK;
D O I：
10.1016/j.apradiso.2015.02.015
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
An Inter-Laboratory Comparison (ILC) study on Dicentric Chromosome Assay (DCA) was carried out between two Indian biodosimetry labs. Human peripheral blood samples exposed to 10 different doses of X-rays up to 5 Gy were shared between the labs to generate calibration data. Validation of calibration curves was done by dose estimation of coded samples exposed to X- or gamma radiation. Reliability of the DCA data for triage application was evaluated by scoring 20, 50 and 100 metaphases in the dose range of 0.5-3.0 Gy. No significant difference was observed between labs regarding the established calibration data as well as the DCA triage dose assessments. Scoring of 20 metaphases (MP) was adequate to detect radiation exposure of > 2 Gy whereas 50 MP were sufficient to determine exposures of 0.5 Gy. Both labs performed the DCA in a reliable manner and made the first step in setting up a biodosimetry network in India. (C) 2015 Elsevier Ltd, All rights reserved.
引用
收藏
页码：77 / 85
页数：9
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