Effect of acute millimeter wave exposure on dopamine metabolism of NGF-treated PC12 cells

被引:10
|
作者
Haas, Alexis J. [1 ,2 ]
Le Page, Yann [1 ,2 ]
Zhadobov, Maxim [2 ,3 ]
Sauleau, Ronan [2 ,3 ]
Le Drean, Yves [1 ,2 ]
Saligaut, Christian [1 ,2 ]
机构
[1] INSERM, Inst Res Environm & Occupat Hlth IRSET, UMR1085, 9 Ave Prof Leon Bernard, F-000 Rennes, France
[2] Univ Rennes 1, F-35000 Rennes, France
[3] IETR, UMR CNRS 6164, F-35000 Rennes, France
关键词
in vitro; 60; GHz; neuron-like model; dopamine turnover; TROUT ONCORHYNCHUS-MYKISS; NERVE GROWTH-FACTOR; CLONAL LINE; WIRELESS COMMUNICATIONS; PHEOCHROMOCYTOMA CELLS; ELECTROMAGNETIC-FIELD; RAT STRIATUM; BRAIN; EXPRESSION; TRANSPORTER;
D O I
10.1093/jrr/rrx004
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Several forthcoming wireless telecommunication systems will use electromagnetic frequencies at millimeter waves (MMWs), and technologies developed around the 60-GHz band will soon know a widespread distribution. Free nerve endings within the skin have been suggested to be the targets of MMW therapy which has been used in the former Soviet Union. So far, no studies have assessed the impact of MMW exposure on neuronal metabolism. Here, we investigated the effects of a 24-h MMW exposure at 60.4 GHz, with an incident power density (IPD) of 5 mW/cmA(2), on the dopaminergic turnover of NGF-treated PC12 cells. After MMW exposure, both intracellular and extracellular contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were studied using high performance liquid chromatography. Impact of exposure on the dopamine transporter (DAT) expression was also assessed by immunocytochemistry. We analyzed the dopamine turnover by assessing the ratio of DOPAC to DA, and measuring DOPAC accumulation in the medium. Neither dopamine turnover nor DAT protein expression level were impacted by MMW exposure. However, extracellular accumulation of DOPAC was found to be slightly increased, but not significantly. This result was related to the thermal effect, and overall, no evidence of non-thermal effects of MMW exposure were observed on dopamine metabolism.
引用
收藏
页码:439 / 445
页数:7
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