Identification of intermediate and product from methemoglobin-catalyzed oxidation of o-phenylenediamine in two-phase aqueous-organic system

被引：6
|
作者：
Li, DJ [1 ]
Li, XW [1 ]
Xie, YX [1 ]
Cai, XQ [1 ]
Zou, GL [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
来源：
BIOCHEMISTRY-MOSCOW | 2005年 / 70卷 / 01期
关键词：
hemoglobin; o-phenylenediamine; intermediate; reaction mechanism;
D O I：
10.1007/s10541-005-0056-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction of phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA. The isolated product and intermediate were characterized by UV-Vis and IR spectrophotometry and HPLC-tandem mass spectrometry. The results showed that the product is 2,3-diaminophenazine, the molecular mass of the intermediate is 212 daltons, and a conceivable structure of the intermediate is suggested. Combining the catalyzed reaction mechanism of peroxidase and our experimental results, a conceivable oxidation reaction mechanism of OPDA and H2O2 using metHb as catalyst is proposed.
引用
收藏
页码：92 / 99
页数：8
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