Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi

被引：37
|
作者：
Oguro, Yoshifumi [1 ]
Yamazaki, Harutake [2 ]
Shida, Yosuke [2 ]
Ogasawara, Wataru [2 ]
Takagi, Masamichi [1 ]
Takaku, Hiroaki [1 ]
机构：
[1] Niigata Univ Pharm & Appl Life Sci, Dept Appl Life Sci, Niigata, Japan
[2] Nagaoka Univ Technol, Dept Bioengn, Niigata, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2015年 / 79卷 / 03期
关键词：
oleaginous yeast; Lipomyces starkeyi; transformation; multicopy integration; RIBOSOMAL DNA; SACCHAROMYCES-CEREVISIAE; VECTOR;
D O I：
10.1080/09168451.2014.982504
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The oleaginous yeast, Lipomyces starkeyi, is an excellent lipid producer with great industrial potential. However, methods for molecular breeding have not been established for L. starkeyi. We describe the development of a system for targeted rDNA integration of multiple copies of a gene into L. starkeyi genome by spheroplast-polyethylene glycol transformation.
引用
收藏
页码：512 / 515
页数：4
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